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Immunological and antioxidant role of curcumin in ameliorating fipronil toxicity in Nile tilapia ( Oreochromis niloticus )
Author(s) -
Abdelkhalek Nevien,
ElAdl Mohamed,
ElAshram Ahmed,
Othman Mayar,
Gadallah Hossam,
ElDiasty Mohamed,
Dawood Mahmoud A.O.,
Almeer Rafa,
Abdel Daim Mohamed
Publication year - 2021
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.15131
Subject(s) - oreochromis , curcumin , biology , nile tilapia , toxicity , antioxidant , glutathione , curcuma , pharmacology , andrology , food science , biochemistry , medicine , fish <actinopterygii> , botany , fishery , enzyme
This study was carried out to investigate the ameliorating effect of dietary curcumin Curcuma longa (CUR) against the subacute toxicity of fipronil (FIP) on Oreochromis niloticus . One hundred and eighty fish were divided into six groups and kept for 30 days; the first group was kept as a control group fed on commercial diet, while the second and third groups were fed on commercial diets supplemented with 1% (CUR1) and 3% (CUR3) curcumin powder/kg diet respectively. The fourth (FIP), fifth (FIP1) and sixth (FIP3) experimental groups were intoxicated with FIP (1/10 96 h LC50), where (FIP1) and (FIP3) groups were fed on a commercial diet supplemented with 1% and 3% of curcumin powder respectively. Hepatorenal damage markers, immunological, tissue antioxidant parameters and anterior kidney expression of IL ‐ 8 , IL ‐ 1β and TGF ‐ β1 genes were determined. Curcumin alleviated the deteriorative effects of FIP intoxication through decreasing hepatic and renal damage markers, improving serum respiratory burst and lysozyme activities. Curcumin provoked a marked decrease in hepatic and renal malondialdehyde and nitric oxide concentration with a significant improvement in tissues' antioxidant status in FIP‐intoxicated fish. Gene expression analysis revealed a significant down‐expression of pro‐inflammatory markers genes after supplementation of curcumin in FIP‐intoxicated fish. In conclusion, the use of 3% curcumin as a feed additive could be implemented to protect fish against the toxic effects of agrochemical wastes via restoring antioxidant and immunological parameters of intoxicated fish.