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Effect of glutamine and cysteine supplementation on quality of cryopreserved sperm of South American silver catfish
Author(s) -
Da Costa Bruna Bitencourt,
Marques Lis Santos,
Lassen Paula Graziela,
Rodrigues Rômulo Batista,
Da RosaSilva Helen Tais,
Moreira José Cláudio Fonseca,
Oliveira Diogo Losch,
Streit Danilo Pedro
Publication year - 2021
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.15070
Subject(s) - glutamine , catfish , biology , glutathione , glutathione peroxidase , cysteine , biochemistry , catalase , sperm , antioxidant , enzyme , amino acid , botany , fishery , fish <actinopterygii>
The aim of this study was to investigate the effects of different concentrations of glutamine (experiment 1) and glutamine + cysteine (experiment 2) on post‐thaw sperm quality of South American silver catfish ( Rhamdia quelen) . The sperm was cryopreserved in cryoprotectant solution (fructose 50 g/L, powdered milk 50 g/L and methanol 100 ml/L) containing different glutamine concentrations (0, 2.5 and 5 mM; experiment 1) and a combination of glutamine (2.5 and 5 mM) and cysteine (2.5 and 5 mM; experiment 2). In experiment 1: glutamine supplementation groups increased DNA fragmentation; the concentration of carbonyl groups was lower and the concentration of sulfhydryl groups was higher in control; a higher activity of superoxide dismutase (SOD), catalase (CAT), glutathione‐S‐transferase (GST) and glutathione peroxidase (GPx) enzymes was observed in the 2.5 and 5 mM groups. In experiment 2: the group with 5 mM of cysteine +5 mM of glutamine presented the higher DNA damage; treatments with 5 mM of glutamine presented the higher concentration of carbonyl groups and a higher activity of CAT and GST. The findings of this study show that glutamine and cysteine, at the concentrations evaluated in this study, does not present satisfactory results but rather damaging effects on sperm quality of R . quelen .