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Bacillus subtilis and Bacillus licheniformis in diets for Nile tilapia ( Oreochromis niloticus ): Effects on growth performance, gut microbiota modulation and innate immunology
Author(s) -
Tachibana Leonardo,
Telli Guilherme Silveira,
Dias Danielle de Carla,
Gonçalves Giovani Sampaio,
Guimarães Mateus Cardoso,
Ishikawa Carlos Massatoshi,
Cavalcante Raissa Bertoncello,
Natori Mariene Miyoko,
Fernandez Alarcon Miguel Frederico,
TapiaPaniagua Silvana,
Moriñigo Miguel Ángel,
Moyano Francisco Javier,
Araújo Ednara Ronise Lima,
RanzaniPaiva Maria José T.
Publication year - 2021
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.15016
Subject(s) - biology , nile tilapia , probiotic , firmicutes , bacillus licheniformis , microbiology and biotechnology , fusobacteria , oreochromis , bacillus subtilis , bacteroidetes , population , proteobacteria , food science , bacteria , fishery , fish <actinopterygii> , genetics , demography , 16s ribosomal rna , sociology
This study aimed to verify the possible positive effects of feeding the commercial probiotic Bioplus 2BC ® [ Bacillus subtilis (1.6 × 10 10 CFU/g) and Bacillus licheniformis (1.6 × 10 10 CFU/g)] to Nile tilapia on its growth performance, nutrient digestibility, gut microbiology, haematology (hematimetric index, erythrocyte, leucocyte and thrombocyte counts) and innate immunology (lysozyme, burst respiratory and phagocyte activities). For this purpose, one in vivo assay was performed following a completely randomized design, with four treatments (0.00%; 0.02%; 0.04%; and 0.08% of probiotic inclusion on ratio) and four replicates. The fish fed with diets containing 0.04% and 0.08% of probiotics presented higher weight gain than the control group, as well as higher thrombocyte counts in the bloodstream. The positive effect of the probiotics on the gut microbiota was illustrated by the higher richness and habitability indexes, soaring genetic variability. On the contrary of control, the groups fed with probiotic had Firmicutes, Fusobacteria and Bacteroidetes phyla, and a lower percentage of Proteobacteria. The inclusion of 0.04% and 0.08% of B. subtilis and B. licherniformis in the fed provides better growth performance and modifies the intestinal microbiota, reducing the class of potential pathogen agent species. The establishment of a beneficial microorganism population may improve host health.