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Deep illumina miRNA sequencing provides insights into the mechanism underlying grass carp reovirus infection
Author(s) -
Ge Ye,
Yao Qiucheng,
Wang Yingying,
Wang Qing,
Zeng Weiwei
Publication year - 2021
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.14904
Subject(s) - biology , microrna , deep sequencing , illumina dye sequencing , grass carp , gene , kegg , computational biology , genetics , gene expression , transcriptome , dna sequencing , fishery , fish <actinopterygii> , genome
Abstract GCRV virulent strain HuNan1307 was infected with healthy grass carp. After 3 weeks, small RNA from kidney tissue of the infected group and the control group was extracted and microarray sequencing was conducted to complete the construction of miRNA libraries of various organs of GCRV virulent strain HuNan1307 and functional annotation of miRNA in the expression profile. Through differential comparison, analysis and verification, miRNAs with many differences between the infection group and the control group were screened out. We found 2,717 miRNAs were detected in the five groups and 14,460 miRNA target genes were predicted and 29 co‐expressions of miRNAs in grass carp infected GCRV with RNA‐Seq. Integrated miRNA expression profiling and pathway analysis show immune effect process, regulation of immune effect process, cell death and regulation of leucocyte‐mediated cytotoxicity, and the PI3K/AKT, Toll‐like and RIG‐I pathways were potentially deregulated. Real‐time quantitative PCR was performed on selected miRNAs in order to validate their expression. Six miRNAs were chosen to verify the result of the deep illumina miRNA sequencing using real‐time PCR method. The result showed that miR710 and miR2696 had the coincide result with deep illumina miRNA sequencing. The development of this study is the basis for understanding the replication, infection, immunity, pathogenesis and transmission of GCRV. This result will help to understand the gene regulation of miRNA between GCRV infection and the host, which contribute to develop new anti‐GCRV drugs.