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Curcumin supplement diet: Enhanced growth and down‐regulated expression of pro‐inflammatory cytokines in Labeo rohita fingerlings
Author(s) -
Leya Tasok,
Dar Showkat A.,
Kumar Gulshan,
Ahmad Irshad
Publication year - 2020
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.14825
Subject(s) - curcumin , biology , aeromonas hydrophila , labeo , lysozyme , immune system , cytokine , pharmacology , andrology , immunology , fish <actinopterygii> , medicine , biochemistry , fishery
The present study was conducted to determine the effect of curcumin supplement diet (CSD) on growth, non‐specific immunity and pro‐inflammatory cytokine gene expression in Labeo rohita fingerlings. 60‐day (0–45 days pre‐challenge and 15 days post challenge; Aeromonas hydrophila ) experiment was conducted for with four treatment groups: T1 (0.5% curcumin level), T2 (1% curcumin level), T3 (1.5% curcumin level), T4 (2% curcumin level) and a control group (0% curcumin level). Kidney tissue samples were collected for RT‐PCR from all the treatment groups at 15 days of interval. After 45 days of feeding, a sub‐sample of fish (15 fish from each replicate) were challenged with a virulent strain of A. hydrophila . The mRNA expression levels of immune‐related genes, viz. tumour necrosis factor‐alpha (TNF‐α), interleukin‐1β (IL‐1β) and Toll‐like receptor‐22 (TLR‐22), were evaluated along with nitro blue tetrazolium (NBT) assay and lysozyme activity. TNF‐α mRNA expression levels were significantly lowered in CSD treatment with the lowest level observed in the T 2 treatment group, followed by T 1 during pre‐challenging period respectively. The IL‐1β and TLR‐22 expression levels also followed the similar trend in different treatment groups. Similarly, NBT and serum lysozyme activities were significantly different among treatment groups. Fish fed with 1.5% level curcumin diet (T3) had significantly highest relative percentage survival (63.64%) compared to the rest of the treatment groups. The results suggested that the diet supplemented with 1.5% curcumin level provides protective effect against A. hydrophila in L. rohita by increasing immunity and reducing the activity of pro‐inflammatory genes.