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Effects of dietary marine microalgae, Tetraselmis suecica , on production, gene expression, protein markers and bacterial count of Pacific white shrimp Litopenaeus vannamei
Author(s) -
Sharawy Zaki Zaki,
Ashour Mohamed,
Abbas Eman,
Ashry Ola,
Helal Mohamed,
Nazmi Hany,
Kelany Mahmoud,
Kamel Abdelwahab,
Hassaan Mohamed,
Rossi Waldemar,
ElHaroun Ehab,
Goda Ashraf
Publication year - 2020
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.14566
Subject(s) - tetraselmis suecica , litopenaeus , biology , shrimp , zoology , food science , glutathione peroxidase , superoxide dismutase , antioxidant , fishery , botany , algae , biochemistry
The marine flagellated Chlorophyta Tetraselmis suecica is among the most important live food species in marine aquaculture. In the present study, the effects of dietary supplementation of dried marine microalgae, Tetraselmis suecica , on growth performance; feed utilization; chemical composition; gene expression of superoxide dismutase (SOD), glutathione peroxidase (GPx) and insulin‐like growth factor 2 (IGF‐II) gene of Pacific white shrimp, Litopenaeus vannamei ; muscle protein polymorphism; and microbial count were assessed and evaluated. Three hundred and sixty L. vannamei (postlarvae) Pls (0.124 ± 0.002 g) were randomly stocked into 40‐L glass aquaria (30 shrimp/aquarium) and fed three times daily four tested diets: a basal diet (control), diet incorporated with 2.5 g kg −1 dried T. suecica (T1), 5 g kg −1 dried T. suecica (T2) and 7.5 g kg −1 dried T. suecica (T3) in triplicates, for 90 days. At the end of the trial, the survival rate (SR) of L. vannamei fed diets supplemented with different levels of T. suecica was significantly ( p < .05) higher than the control diet. The highest weight gain and specific growth rate and the best feed conversion ratio were recorded on L. vannamei fed a diet supplemented with a 7.5 g/kg dried T. suecica . The highest protein, lipid and ash contents were obtained in L. vannamei fed the diet containing 7.5 g/kg T . suecica , when compared with the remaining tested diets. The gene expression of antioxidant genes SOD and GPx was the lowest in the T3 group in comparison with the control group. Meanwhile, expression level of IGF‐II was higher in the T2 group. The total heterotrophic bacterial count was significantly ( p < .05) increased with the cumulative T. suecica level, while no significant ( p > .05) differences were found in the total Vibrio count among treatments. Overall, the present results have shown that the diet supplemented with the highest inclusion level of dried T. suecica resulted in improved growth and nutrient utilization.