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Effect of Dimethyl sulfoxide (DMSO) and egg yolk on sperm motility, fertility and hatching rates of depik Rasbora tawarensis (Pisces: Cyprinidae) eggs after short‐term cryopreservation
Author(s) -
Muchlisin Zainal A.,
Sarah Putri I.,
Aldila Dhea F.,
Eriani Kartini,
Hasri Iwan,
Batubara Agung S.,
Nur Firman M.,
Mustaqim Mustaqim,
Muthmainnah Cut Ruhul,
Abinawanto Abinawanto,
Wilkes Martin
Publication year - 2020
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.14516
Subject(s) - cryoprotectant , dimethyl sulfoxide , yolk , cryopreservation , biology , hatching , sperm , human fertilization , andrology , anatomy , zoology , botany , food science , fishery , embryo , chemistry , organic chemistry , medicine
Cryoprotectant is the crucial factor in the cryopreservation process. In general, there are two types of cryoprotectant, permeating and non‐permeating cryoprotectants. Dimethyl sulfoxide (DMSO) and egg yolk are common permeating and non‐permeating cryoprotectants respectively. Hence, the objective of the present study was to determine the best proportion of DMSO and egg yolk for the cryopreservation of Rasbora tawarensis sperm. A completely randomized experimental design was used in this study which involves two types of cryoprotectant and their combination at different concentrations, namely 5% DMSO, 5% egg yolk, 5% DMSO + 5% egg yolk and 2.5% DMSO + 2.5% egg yolk. Every treatment was conducted in three replicates. Combination of 5% DMSO + 5% egg yolk gave the best results cryoprotectant treatment had significant effects on sperm motility, fertilization and hatching rate of the R. tawarensis eggs ( p < .05). It is concluded that the best proportion of cryoprotectants for sperm cryopreservation in this species is 5% DMSO + 5% egg yolk.