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Thiamine requirement of Chinese soft‐shelled turtle, Pelodiscus sinensis (Wiegmann)
Author(s) -
Tsai TzuHsuan,
Huang ChenHuei
Publication year - 2019
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.14344
Subject(s) - thiamine , biology , turtle (robot) , zoology , vitamin , body weight , endocrinology , medicine , biochemistry , ecology
The objective of the present study was to estimate the dietary thiamine (vitamin B 1 ) requirement of juvenile soft‐shelled turtles, Pelodiscus sinensis . Eight experimental diets containing 0, 1.90, 3.63, 5.65, 7.51, 9.62, 11.37 and 13.64 mg thiamine/kg diet were fed to 160 soft‐shelled turtles reared individually for 10 weeks. The turtles had an average weight of 5.33 ± 0.21 g. Among all the dietary groups, weight gain, feed utilization and tissue thiamine were the lowest in the turtles fed with a thiamine‐free diet. The variables increased when dietary thiamine increased and then levelled off beyond 3.63 mg/kg diet. In contrast, plasma pyruvate and lactate concentrations in turtles decreased when dietary thiamine increased and then levelled off beyond 3.63 and 5.65 mg/kg diet respectively. Estimation using a broken‐line regression model, the vitamin B 1 requirement of soft‐shelled turtles was 3.2 mg thiamine/kg diet based on weight gain and plasma lactate concentration. When tissue thiamine and plasma pyruvate concentrations were used as the indicators, the estimated requirement was 5.4–6.5 mg/kg diet.