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Evaluation of a cocktail of phages for the control of presumptive Vibrio parahaemolyticus strains associated to acute hepatopancreatic necrosis disease
Author(s) -
Makarov Román,
LomelíOrtega Carlos Omar,
ZermeñoCervantes Lina Angélica,
GarcíaÁlvarez Eugenio,
GutiérrezRivera Jesús Neftalí,
CardonaFélix César Salvador,
MartínezDíaz Sergio Francisco
Publication year - 2019
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.14258
Subject(s) - vibrio parahaemolyticus , biology , brine shrimp , lytic cycle , microbiology and biotechnology , outbreak , virulence , shrimp , phage therapy , vibrio , bacteriophage , virology , bacteria , virus , fishery , gene , biochemistry , genetics , escherichia coli
Shrimp culture is a well‐established and fast‐growing industry that produces economic and social benefits in many countries. However, during the last years, it was severely affected by the emergence of the Early Mortality Syndrome (EMS) or Acute Hepatopancreatic Necrosis Disease (AHPND). This disease is mainly attributed to Vibrio parahaemolyticus , and currently, there is no effective cure or treatment. In this study, the use of T2A2 and VH5e bacteriophages was evaluated to control different AHPND‐positive strains (presumptively identified as V. parahaemolyticus, VP AHPND ) under laboratory conditions. Lytic effect of T2A2 and VH5e bacteriophages against different strains isolated from AHPND outbreaks was corroborated. In addition, the effectiveness of the mixture of both phages was tested on a brine shrimp experimental infection model using three highly virulent VP AHPND strains. It has been found that phage‐treated brine shrimp had significantly higher survival percentage compared with non‐treated groups ( p < .001). Also, phage cocktail was found to be harmless to the organisms. These results suggest that the phage mixture is worth considering as a possible control measure for positive AHPND strains, although it is clear that further and more extensive testing is needed.