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The protective effects of Vitamin C and Trolox on kinematic and oxidative stress indices in rainbow trout sperm cells against flower‐like ZnO nanoparticles
Author(s) -
Özgür Mustafa Erkan
Publication year - 2019
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.14236
Subject(s) - trolox , oxidative stress , sperm , antioxidant , biology , vitamin c , vitamin e , rainbow trout , lipid peroxidation , andrology , materials science , medicine , endocrinology , biochemistry , botany , antioxidant capacity , fishery , fish <actinopterygii>
This study investigated the protective effects of 1 mM Vitamin C and 1 mM Trolox on kinematic and oxidative stresses in rainbow trout ( Oncorhynchus mykiss ) sperm cells against flower‐like zinc oxide nanoparticles (ZnO‐NP, 1 mg/L) in vitro , during 2 hr. Trolox showed protective effects on all kinematic parameters measured for sperm cells against ZnO‐NPs, whereas Vitamin C only affected angular path velocity (VAP), linearity (LIN) and amplitude of lateral displacement of the sperm cell head (ALH). Although ZnO‐NPs reduced total glutathione (TGSH) and catalase (CAT) compared with the control, there were positive treatment effects for both Trolox and Vitamin C on antioxidant capacity. Conversely, while ZnO‐NP increased the rate of malondialdehyde (MDA) lipid peroxidation, it decreased following antioxidant therapy. This research suggests that Trolox and Vitamin C play a protective role for rainbow trout sperm exposed to ZnO‐NP.