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Characterization of a novel marine origin aerobic nitrifying–denitrifying bacterium isolated from shrimp culture ponds
Author(s) -
Zhao Kun,
Tian Xiangli,
Li Haidong,
Dong Shuanglin,
Jiang Wenwen
Publication year - 2019
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.14055
Subject(s) - denitrifying bacteria , biology , nitrite , ammonium , ammonium chloride , bacillus subtilis , shrimp , denitrification , nitrifying bacteria , nitrate , aerobic denitrification , substrate (aquarium) , nuclear chemistry , chromatography , bacteria , nitrogen , nitrification , chemistry , fishery , ecology , organic chemistry , genetics
A novel marine origin Bacillus subtilis strain H1 isolated from a shrimp culture pond effectively removed NH 4 + ‐N, NO 2 - ‐N and NO 3 - ‐N, with a maximum ammonium, nitrite and nitrate removal rate of 2.35 mg NH 4 + ‐N L −1 hr −1 per OD, 9.64 mg NO 2 - ‐N L −1 hr −1 per OD and 0.75 mg NO 3 - ‐N L −1 hr −1 respectively. The gas chromatography–isotope ratio mass spectrometry results indicated that N 2 O was emitted when 15 NH 4 Cl, Na 15 NO 2 or Na 15 NO 3 was used. Additionally, N 2 was also produced when Na 15 NO 2 was used. Single‐factor experiments suggested that the optimal conditions for NH 4 + ‐N and NO 2 - ‐N removal were glucose as a carbon source, C/N 15, initial pH 7.5, 30 g/L NaCl, 28°C and a shaking speed of 160 rpm. Orthogonal tests showed that the optimal conditions for NH 4 + ‐N removal were C/N 15, pH 9, 10 g/L NaCl and shaking speed 160 rpm when ammonium chloride was used as the substrate. The optimal conditions for NO 2 - ‐N removal were C/N 10, pH 6, 10 g/L NaCl and a shaking speed of 160 rpm when sodium nitrite was used as the substrate. In summary, B. subtilis strain H1 had highly efficient aerobic nitrifying–denitrifying ability and high adaptability, suggesting that it is potentially valuable to marine aquaculture.