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Pharmacokinetic/pharmacodynamic properties and P‐glycoprotein expression in pacific white shrimp, Litopenaeus vannamei after oral administration at three dosages of oxolinic acid
Author(s) -
Fu Guihong,
Yin Guifang,
Wang Yuan,
Zhou Junfang,
Zhao Shu,
Li Xincang,
Fang Wenhong
Publication year - 2019
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.14037
Subject(s) - cmax , dose , shrimp , oxolinic acid , litopenaeus , biology , pharmacokinetics , oral administration , pharmacodynamics , minimum inhibitory concentration , zoology , pharmacology , microbiology and biotechnology , antibiotics , fishery , nalidixic acid , antibiotic resistance
The experiments explored the pharmacokinetics (PK) properties of oxolinic acid (OXA) after oral administration at three dosages (10, 30 and 80 mg/kg) via medicated feed in the shrimp. The results showed that the C max values of 4.31, 14.93 and 16.62 mg/L and AUC 0–∞ values of 92.61, 252.30 and 364.27 mg hr −1 L −1 were observed at three OXA dosage groups in the haemolymph respectively. In the hepatopancreas, C max values of 7.90, 27.23 and 60.51 mg/kg and AUC 0–∞ values of 42.01, 133.06 and 219.06 mg hr −1 L −1 were observed at 0.5 hr post administration respectively. In the muscle, C max values of 1.62, 5.80 and 7.36 mg/kg and the AUC 0–∞ values of 25.64, 98.10 and 134.24 mg hr −1 L −1 were observed at 2 hr post administration respectively. In the gills, C max values of 2.87, 8.08 and 12.12 mg/kg and the AUC 0–∞ values of 51.38, 118.65 and 206.48 mg hr −1 L −1 were observed at 4 hr post administration respectively. In addition, the in vitro MIC values of OXA at three dosages against 132 strains of Vibrio were examined and showed that the minimum inhibitory concentration (MIC) values for OXA primarily ranged from 0.15–1.25 µg/ml, including eight strains of Vibrio showing MIC values ≥5 µg/ml. The MIC 50 and MIC 90 values of 132 strains were 0.62 and 1.25 μg/ml respectively. The AUC 0–24 /MIC 90 ratios of Vibrio were 140.4 in 30 mg/kg group. Furthermore, the P‐glycoprotein (P‐gp) expression was determined in shrimp tissues after administration to three dosage groups (10, 30 and 80 mg/kg). The results showed that P‐gp expression was up‐regulated in the hepatopancreas (5.36‐, 13.68‐ and 31.06‐fold respectively) compared with the control group.