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A novel enzymatic pre‐treatment improves amino acid utilization in feather meal fed to rainbow trout ( Oncorhynchus mykiss )
Author(s) -
Pfeuti Guillaume,
Cant John P.,
Shoveller Anna K.,
Bureau Dominique P.
Publication year - 2019
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.14021
Subject(s) - biology , feather meal , feather , rainbow trout , distilled water , zoology , lysine , arginine , fish meal , factorial experiment , bioavailability , completely randomized design , amino acid , meal , soybean meal , food science , biochemistry , fishery , chromatography , fish <actinopterygii> , chemistry , ecology , pharmacology , raw material , statistics , mathematics
A growth trial followed by a digestibility trial were conducted to assess the bioavailability of arginine (Arg) and amino acid digestibility of two feather meals (FeMs) and their pre‐treated counterparts (PTFeMs). Two FeMs, identified as FeM1 and FeM2, were incubated in 200% distilled water (%FeM w/w), with 0.05% of Savinase ® 16L (%FeM v/w) and 2% sodium sulphite (%FeM w/w) at 55ºC for 24 hr in a shaking incubator to produce PTFeM1 and PTFeM2 respectively. A basal diet, highly deficient in Arg (12.0 g/kg Arg) was formulated and 10 other diets were formulated to contain 13.5 or 15.0 g/kg Arg by adding increasing amounts of L‐Arg, FeMs or PTFeMs. The experimental diets were fed for 8 weeks using a standard protocol. At 13.5 g/kg Arg, the PTFeMs supported 10.5% to 11.5% higher growth rates ( p < 0.05) than their untreated counterparts. The diets formulated with PTFeMs presented 3.5%–4.5% and 2.7%–5.9% higher ( p < 0.05) apparent digestibility coefficients of protein when compared to the diets containing the FeM counterparts at 13.5 and 15.0 g/kg Arg respectively. The results from this study demonstrate the potential of the pre‐treatment to improve the nutritive value of FeMs in rainbow trout.