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Improvement of reproductive indices, lysozyme activity, and disease resistance in live‐bearing ornamental fish, Poecilia latipinna using Artemia supplementation with treated yeast cell, Saccharomyces cerevisiae
Author(s) -
Rezaei Aminlooi Vahid,
Ahmadifard Nasrollah,
Tukmechi Amir,
Agh Naser
Publication year - 2019
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13869
Subject(s) - biology , poecilia , aeromonas hydrophila , yeast , lysozyme , zoology , fecundity , food science , microbiology and biotechnology , fishery , fish <actinopterygii> , population , biochemistry , demography , sociology
In the present study, the effects of Artemia supplemented with 2‐β‐mercapto‐ethanol (β‐ME) treated yeast cell, Saccharomyces cerevisiae, on growth and reproductive performance, lysozyme activity, and disease resistance to Aeromonas hydrophila of freshwater ornamental species, Poecilia latipinna, were investigated. Within 60 days, molly fish were fed with three treatments including commercial food (T1), un‐supplemented Artemia (T2), and Artemia supplemented with β‐ME‐treated yeast cell (at concentration of 4 × 10 7 CFU/L of water) (T3). After the feeding period, the fish were exposed to 100 μl of a suspension (1.1 × 10 7 cells/ml) of A. hydrophila (BCCM5/LMG3279) and the cumulative mortality rates were recorded for 12 days. No significant difference was found between survival rate and growth performance of P. latipinna except for weight gain that was higher in fish fed through Artemia supplemented with β‐ME‐treated yeast cell compared to control group. Fecundity rate was significantly improved in fish fed using T3 with the maximum amount of 49.5 ± 2.29 per female ( p < 0.05). Besides, lysozyme activity was significantly increased in group 3 ( p < 0.05). Moreover, lowest fish mortality was significantly observed in this treatment ( p < 0.05). In addition, the number of colonies formed by yeast cell in T3 (634 × 10 3 CFU/g intestine) showed significant difference with other treatments ( p < 0.05). In sum, Artemia enriched with β‐ME‐treated yeast improved reproductive indices, immune responses, and resistance against A. hydrophila of P. latipinna .