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Glucose tolerance of grass carp Ctenopharyngodon idellus after a long‐term adaptation to carbohydrate‐to‐lipid ratio diets
Author(s) -
Li RuiXin,
Liu HongYu,
Chen Qiang,
Tan BeiPing,
Dong XiaoHui,
Chi ShuYan,
Yang QiHui,
Zhang Shuang,
Chen LiQiao
Publication year - 2018
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13856
Subject(s) - glycogen , biology , grass carp , medicine , phosphoenolpyruvate carboxykinase , endocrinology , carbohydrate , glucokinase , glycogen synthase , gluconeogenesis , carbohydrate metabolism , biochemistry , fish <actinopterygii> , metabolism , enzyme , diabetes mellitus , fishery
Unlike the case of mammals, teleost fish generally have poor ability to utilize carbohydrate, and the clearance of glucose from the plasma is sluggish. In this study, after a 8‐week feeding trial, we conducted a glucose tolerance test to hypothesize that the capability of grass carp ( Ctenopharyngodon idellus ) to clear a glucose load is associated with its nutrient history of different carbohydrate‐to‐lipid ratio diets (CHO:L: 1.52%, 5.84% and 34.84%) through glycogen synthesis and glucose metabolic key enzymes. Our results showed that after a glucose load, the plasma glucose of fish fed with high CHO:L‐ratio diet (34.84%) first declined to baseline conditions, and the peak value of liver and muscle glycogen content was significantly higher in high CHO:L‐ratio but lower in low CHO:L group (1.52%). In addition, after glucose administration, hepatic GK (glucokinase) activity of fish fed with high CHO:L‐ratio diet increased more significantly than that of the other groups, and PEPCK (phosphoenolpyruvate carboxykinase) activity obviously decreased in low CHO:L‐ratio group. The results indicated that a long‐term adaptation to the high CHO:L‐ratio diet can improved the glucose tolerance of C. idellus through the promotion of glycogen synthesis and GK activity.