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Improved healing of the deeply incisional wounds in partially scaled common carp by zinc sulphate bath
Author(s) -
ElAdl Mohamed,
Abdelkhalek Nevien,
Mahgoub Hebatallah A.,
Salama Mohamed F.,
Ali Mayar
Publication year - 2018
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13805
Subject(s) - wound healing , superoxide dismutase , vascular endothelial growth factor , glutathione , catalase , malondialdehyde , biology , antioxidant , andrology , angiogenesis , common carp , fibroblast , transforming growth factor , zinc , endocrinology , chemistry , biochemistry , medicine , immunology , vegf receptors , cancer research , fishery , fish <actinopterygii> , organic chemistry , in vitro , enzyme , cyprinus
This study aimed to investigate the role of zinc immersion bath in promoting wound‐healing process in partially scaled common carp using different concentrations of zinc sulphate (ZnSO 4 ). After wound induction, three different doses of ZnSO 4 were used (1/20, 1/10 and 1/5 of the calculated LC50) for 10 days. After the end of the experimental period, tissue samples were collected from the area surrounding the wound for analysing the expression of the following genes: collagen type I alpha 1 ( COL1α1 ), transforming growth factor ( TGF )‐ β1 , vascular endothelial growth factor ( VEGF ) ‐A , metallothionein (Met) and fibroblast growth factor (FGF)‐ 7. Moreover, the tissue antioxidant markers including superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH) and malondialdehyde (MDA) were also assessed. The extent of the healing process was also assessed by histopathological examination. The results revealed that the use of ZnSO 4 at 1/10 of the LC50 caused a significant improvement in tissue reepithelization, angiogenesis and tissue remodelling through overexpression of Col1α1 , TGF‐β1 , VEGF‐A and FGF‐7 . Moreover, a significant improvement in antioxidant markers was also observed with ZnSO 4 immersion. In conclusion, this study showed that ZnSO 4 immersion at 1/10 of the calculated LC50 improved the healing of the incisional wound in partially scaled common carp via upregulation of the examined growth factor genes and improvement of the antioxidant status of fish.