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Iron bioavailability of different sources in juvenile grouper Epinephelus coioides
Author(s) -
Huang QinCheng,
Wang ErLong,
Kwaku Amoah,
Dong XiaoHui,
Tan BeiPing,
Chi ShuYan,
Yang QiHui,
Zhang Shuang,
Liu HongYu,
Yang YuanZhi
Publication year - 2018
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13742
Subject(s) - epinephelus , bioavailability , zoology , grouper , biology , arsenic , significant difference , food science , chemistry , medicine , fishery , fish <actinopterygii> , organic chemistry , bioinformatics
Abstract A 70‐day experiment was implemented to study the iron (Fe) bioavailability of three Fe sources in Epinephelus coioides (initial weight, 12.19 ± 0.14 g). Treatments consisted of 0, 50, 90, 130, 170 and 210 mg supplemental Fe/kg from iron sulphate (Fe SO 4 ), ferric citrate (Fe‐citrate) and hydroxy methionine analogue iron (Fe‐ MHA ). The results showed that the growth performance was not affected by Fe level regardless of Fe sources. Hepatic catalase activity ( CAT ), the haematocrit value (Hct), haemoglobin (Hb), mean corpuscular volume ( MCV ) and Fe content in whole body or liver firstly increased significantly and then plateaued as Fe level increased regardless of the Fe source, except significant decreasing of Hct in 210 mg Fe/kg group from Fe SO 4 . Manganese (Mn) content decreased significantly and then kept stable in the whole body and liver whatever the source was. The slope‐ratio method using Fe SO 4 as the reference and Hb content, CAT activity, body and hepatic Fe concentration as the response parameter showed the average relative bioavailability of Fe‐citrate and Fe‐ MHA was 103.25 ± 4.35 ( n = 4) and 134.5 ± 15.67 ( n = 4), respectively, with the maximum value of Fe‐ MHA in any case. In conclusion, Fe‐ MHA was the most effective to meet preferable haematological index, CAT activity and mineral deposition, with little difference between Fe‐citrate and Fe SO 4 , athough Fe levels with different sources failed to influence the growth in grouper.