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Pharmacokinetics of minocycline in crucian carp ( Carassius auratus ) after intravenous and oral administration
Author(s) -
Yang Fan,
Yang Fang,
Yang Yurong,
Kong Tao,
Wang Guoyong,
Bai Dongying
Publication year - 2018
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13739
Subject(s) - crucian carp , pharmacokinetics , volume of distribution , minocycline , bioavailability , body weight , oral administration , absorption (acoustics) , biology , pharmacology , antibiotics , zoology , endocrinology , fish <actinopterygii> , microbiology and biotechnology , materials science , fishery , composite material
The pharmacokinetic of minocycline was studied after a single intravenous as well as oral dose (5 mg/kg body weight) in crucian carp ( Carassius auratus ) reared in freshwater at 10°C. Plasma samples were randomly collected from six fish at each sampling time. Plasma concentrations were determined by high‐performance liquid chromatography and further subjected to noncompartmental analysis. Initial concentration of minocycline just after intravenous administration was calculated as 7.320 μg/ml, while the other parameters after intravenous injection were determined as flows: apparent elimination rate constant ( λ z ) of 0.064 per hr, apparent elimination half‐life ( t 1 / 2 λ z) of 10.82 hr, total body clearance (Cl) of 142.72 ml/hr/kg, volume of distribution (Vz) of 2,227.38 ml/kg and volume of distribution at steady‐state (Vss) of 1,937.08 ml/kg. While after oral administration, the λ z , t 1 / 2 λ z, mean absorption time ( MAT ), absorption half‐life (t 1/2ka ) and bioavailability were determined as 0.059 per hr, 11.74, 5.55, 3.84 hr, and 81.98%, respectively, and the peak concentration was observed as 1.474 ± 0.362 μg/ml at 8 hr. It was shown that minocycline was slowly but relatively completely absorbed, extensively distributed, and slowly eliminated in crucian carp. Based on the ratios of AUC 0–24 hr / MIC 90, a minocycline dosage of 5 mg/kg body weight administered intravenously or orally would be only effective to successfully treat crucian carp infected by bacterium with MIC values ≤0.25 μg/ml.