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Genomic structure and molecular characterization of growth hormone and its expression response to different feed types in golden pompano Trachinotus ovatus (Linnaeus, 1758)
Author(s) -
Liang YinYin,
Guo HuaYang,
Zhu KeCheng,
Zhang Nan,
Yang JingWen,
Sun XiaoXiao,
Jiang ShiGui,
Zhang DianChang
Publication year - 2018
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13653
Subject(s) - biology , open reading frame , gene , exon , feed conversion ratio , gene expression , genetics , zoology , endocrinology , body weight , peptide sequence
Growth hormone and feed types all play important roles in the growth of vertebrates. In this study, to investigate molecular characterization of the Trachinotus ovatus growth hormone ( To GH ) and its expression in response to the different feed types, we characterized the genomic structure of GH and analysed expression patterns using quantitative real‐time PCR ( qRT ‐ PCR ). To GH spans approximately 4.0 kb and consists of six exons and five introns, with 606 bp open reading frame ( ORF ). The 5′‐flanking region contains consensus sequences for three TATA boxes, one pit‐1a, three GRE , two TRE , two HNF ‐3 and two ERE . A phylogenetic analysis of Percoidei confirmed that the phylogenetic trees based on the GH gene are reliable at the genera level. The expression of To GH mRNA was highest in intestine, brain and muscle and lowest in liver, spleen and kidney ( p < .05). The dietary experiment demonstrated that the transcription level of To GH in T. ovatus fed with iced fish was higher than in those fed with iced squid and pelleted feed ( p < .05). Fish fed with pelleted feed had higher To GH expression in their intestine than those fed iced squid ( p < .05); however, To GH expression in fish fed with iced fish did not significantly differ from the other feeding groups. This study would be useful to understanding the potential function of GH gene in out pituitary tissues by diets and providing a certain strategy for feed research.