Premium
Molecular cloning, characterization, tissue expression and nutritional regulation of O‐Glc NA c transferase gene in hybrid grouper ( Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂ )
Author(s) -
Li Songlin,
Zhang Jiacan,
Sang Chunyan,
Li Ziqiang,
Chen Naisong,
Huang Xuxiong
Publication year - 2018
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13533
Subject(s) - biology , grouper , gene , genbank , transferase , untranslated region , cloning (programming) , molecular cloning , gene expression , biochemistry , coding region , microbiology and biotechnology , messenger rna , enzyme , fishery , fish <actinopterygii> , computer science , programming language
O‐Glc NA c transferase gene ( OGT ) was considered as the sole rate‐limiting enzyme in the O‐Glc NA c modification. In the present study, the OGT gene of hybrid grouper ( Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂) was cloned and characterized, and its expression in response to dietary carbohydrate level and acute glucose treatment was investigated. The full‐length of OGT (GenBank accession no. KY656469 ) was 4,063 bp, including a 302 bp 5′untranslated terminal region ( UTR ), a 3,165 bp coding region that encoded 1,054 amino acids residues and a 596 bp 3′ UTR . The highly conservation of OGT gene between fish and mammals was also observed through multiple sequences alignment and phylogenetic analysis. O‐Glc NA c transferase gene was ubiquitously expressed in all detected tissues with highest expressions in brain and liver, to a lesser degree, in eye, heart, kidney and intestine. The increasing dietary carbohydrate from 8.02% to 16.08% had no significant effect on the mRNA expression of OGT . However, the expression of OGT was slightly elevated at 6 hr post‐glucose injection, and the elevation became significant at 24 hr time‐point. These data may enhance our understanding on the nutritional regulation of OGT and O‐Glc NA c modification in fish species.