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Effects of guanidinoacetic acid supplementation in all‐plant protein diets on growth, antioxidant capacity and muscle energy metabolism of bullfrog Rana ( Lithobates ) catesbeiana
Author(s) -
Zeng QiuHui,
Rahimnejad Samad,
Wang Ling,
Song Kai,
Lu Kangle,
Zhang ChunXiao
Publication year - 2018
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13505
Subject(s) - bullfrog , biology , malondialdehyde , lactate dehydrogenase , creatine kinase , glycogen , medicine , zoology , catalase , lithobates , endocrinology , meal , antioxidant , biochemistry , food science , enzyme
Effects of guanidinoacetic acid ( GAA ) supplementation in all‐plant protein diets were evaluated on growth, antioxidant capacity and muscle energy metabolism of Rana ( Lithobates ) catesbeiana . Six diets were prepared: a basal fish meal diet ( FM ), an all soybean meal diet ( SM ) and four GAA ‐supplemented diets by adding 0.2, 0.4, 0.6 and 0.8 g GAA /kg to the SM diet ( GAA 2, GAA 4, GAA 6 and GAA 8 diets). Triplicate groups of bullfrog (45 ± 0.2 g) were fed the diets to apparent satiation for 8 weeks. Total substitution of FM with SM led to significantly ( p < .05) reduced growth and feed utilization. Adding 0.4 g GAA /kg to the SM diet significantly improved growth performance, and the values were comparable to those of FM group. SM and GAA 2 groups showed drastically lower serum total antioxidant capacity than FM , GAA 4 and GAA 6 groups. Also, SM and GAA 2 groups showed significantly lower catalase activity than FM and GAA 8 groups. A remarkable increase in serum malondialdehyde concentration was detected in SM and GAA 2 groups. FM and GAA 6 groups exhibited significantly higher serum creatinine concentration than other groups. Muscle creatine kinase activity and glycogen content were significantly decreased in SM group and application of 0.4–0.6 g GAA /kg significantly improved their values. GAA 4 group showed significantly lower pyruvate kinase activity than FM and GAA 6 groups. Also, GAA 2 and GAA 4 groups had significantly lower succinate dehydrogenase (SDH) activity than other treatments. These findings show that supplementing 0.4 g/kg GAA to SM ‐based diets improves growth, antioxidant capacity and muscle energy metabolism.