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Cytogenetic analysis of hybrids and hybrid triploids between the river puffer, Takifugu obscurus , and the tiger puffer, Takifugu rubripes
Author(s) -
Yoo Gwang Yeol,
Lee Tae Ho,
Gil Hyun Woo,
Lim Sang Gu,
Park InSeok
Publication year - 2018
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13493
Subject(s) - takifugu rubripes , biology , hybrid , zoology , midgut , metaphase , anatomy , chromosome , botany , fishery , genetics , larva , fish <actinopterygii> , gene
Cytogenetic analyses of the river puffer, Takifugu obscurus , the tiger puffer, Takifugu rubripes and hybrids produced between female T. obscurus and male T. rubripes and their hybrid triploids (produced by cold shock treatment at 4°C) were performed. T. obscurus had 2 n = 44 chromosomes and 1.84 ± 0.019 pg DNA /cell, T. rubripes had 2 n = 44 and 2.64 ± 0.015, the hybrids had 2 n = 44 and 2.15 ± 0.010 and the hybrid triploids had 3 n = 66 and 3.22 ± 0.010. The erythrocyte values of the hybrids were more similar to those for T. obscurus , whereas the hepatocyte, midgut and proximal tubule kidney cell values of the hybrids fell down between those for the parental species ( p < .05). The erythrocyte, proximal tubule, hepatocyte and midgut epithelial cell sizes for the hybrid triploids were 1.5‐fold larger than those for the hybrids ( p < .05). The thickness of retina and each layer in the hybrid triploids were 1.1‐fold larger than those of the hybrids ( p < .05) and did not differ significantly among T. obscurus , T. rubripes and the hybrids ( p > .05); however, the hybrid triploids had fewer cell nucleus outer layers than the hybrids ( p < .05). Gonad development in the hybrids and hybrid triploids was less matured than in T. obscurus and T. rubripes . The metaphase nucleolus organizer regions ( NOR s) and the gill cells of T. obscurus , T. rubripes and the hybrids contained two satellite telocentrics, whereas the hybrid triploids contained three satellite telocentrics.