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Genetic identification of a newly synthetic allopolyploid strain with 206 chromosomes in polyploid gibel carp
Author(s) -
Lu Meng,
Wang ZhongWei,
Hu ChongJiang,
Zhou Li,
Gui JianFang
Publication year - 2018
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13485
Subject(s) - biology , polyploid , carp , genetics , strain (injury) , clone (java method) , chromosome , ploidy , genome , sperm , karyotype , gene , fish <actinopterygii> , anatomy , fishery
Abstract A newly synthetic allopolyploid strain ( SAS ) was selected and established from gynogenetic offspring of gibel carp clone A + with 156 chromosomes induced by common carp sperm with 50 chromosomes. In this study, the allopolyploid strain was detected to contain 206 chromosomes, and the growth trait was evaluated to have 25.15% growth faster than that of clone A + . Genetic marker analyses of transferrin ( Tf ) alleles, ITS 1 sequences and mt DNA D‐loop sequences indicated that the allopolyploid strain was synthetized from maternal gibel carp clone A + and paternal common carp, and the synthetic chromosome sets were further confirmed by genomic in situ hybridization (GISH) and chromosome localization of 45S rDNA . Significantly, the synthetic allopolyploid strain has tended to be stable by five successive generations of gynogenesis, because it still keeps unisexual reproduction mode of gynogenesis. Therefore, it will become a novel variety for gibel carp aquaculture in future.