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Molecular isolation and characterization of a haemocyanin of Macrobrachium rosenbergii reveal its antibacterial activities
Author(s) -
Srisuk Chutima,
Senapin Saengchan,
Bendena William G,
Longyant Siwaporn,
Sithigorngul Paisarn,
Chaivisuthangkura Parin
Publication year - 2018
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13481
Subject(s) - biology , macrobrachium rosenbergii , penaeus monodon , shrimp , open reading frame , microbiology and biotechnology , hemolymph , edwardsiella tarda , gene , peptide sequence , prawn , biochemistry , fishery
Haemocyanin is a multi‐subunit protein complex found in the haemolymph and is involved in the immune system of crustaceans. In this study, a haemocyanin gene of Macrobrachium rosenbergii , designated MrHc , was successfully isolated. The MrHc gene contained an open reading frame ( ORF ) of 1,992 nucleotides, encoding a protein of 663 amino acid residues with a molecular mass of 76.5 kD a. The deduced amino acid sequence contained distinct structural motifs of the haemocyanin superfamily, including an all‐alpha domain, a copper‐containing domain and an immunoglobulin‐like domain. Based on the phylogenetic analysis, the Mr HC protein demonstrated a close relationship with the haemocyanins of Palaemon carinicauda and Macrobrachium nipponense . The MrHc gene was expressed in various shrimp tissues, including the hepatopancreas, gill, haemocytes, stomach and muscle. After Macrobrachium rosenbergii nodavirus ( Mr NV ) challenge tests, the MrHc gene was up‐regulated 237‐fold at day 2. A recombinant protein of the MrHc immunoglobulin‐like domain exhibited antibacterial activity against Vibrio vulnificus , V. parahaemolyticus , Aeromonas caviae , A. veronii , A. hydrophila and Bacillus cereus . This study suggested that MrHc may play important roles in the shrimp innate immune response to Mr NV infection and bacterial infection.