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Effects of potential probiotic Bacillus subtilis KADR1 and its subcellular components on immune responses and disease resistance in Labeo rohita
Author(s) -
Ramesh Dharmaraj,
Souissi Sami
Publication year - 2018
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13467
Subject(s) - aeromonas hydrophila , biology , labeo , microbiology and biotechnology , immune system , lysozyme , bacillus subtilis , respiratory burst , probiotic , immunity , aeromonas , immunology , bacteria , fish <actinopterygii> , fishery , genetics
The present study was conducted to determine the effects of Bacillus subtilis KADR 1 and its subcellular components on immunity and disease resistance in Labeo rohita against Aeromonas hydrophila infection. Fish were fed diet containing different concentrations of live bacterial cells ( DI ‐10 6 , DII ‐10 8 and DIII ‐10 10 CFU /g) and another group of fish were immunized intraperitoneally with cellular components ( WCP s, CWP s and ECP s) of Bacillus subtilis KADR 1. After 4 weeks of trial, fish were challenged intraperitoneally with Aeromonas hydrophila cell suspension and survival percentage was recorded. Significantly higher post‐challenge survivability was recorded in fish groups fed 10 8 CFU /g of KADR 1 (80.24%; RPS = 75.76%) or immunized with WCP s (77.77%; RPS = 72.73%), compared with the control (18.51%). Analysis of immunological parameters viz. serum lysozyme, phagocytosis, serum total protein, respiratory burst, serum IgM levels, superoxide dismutase and alternative complement pathway activity reflected significant enhancement ( p < .05) of immune response in fish fed 10 6 , 10 8 and 10 10 CFU /g of live cells, or immunized with cellular components of Bacillus subtilis KADR 1, with the highest values were observed DII fed group, followed by the group immunized with WCP s. Our results suggest that dietary administration of Bacillus subtilis KADR 1 at 10 8 CFU /g can effectively enhance the immune responses and disease resistance in Labeo rohita against Aeromonas hydrophila .