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Evaluation of some intestinal cytokines genes expression and serum innate immune parameters in common carp ( Cyprinus carpio ) fed dietary loquat ( Eriobotrya japonica ) leaf extract
Author(s) -
Hoseinifar Seyed Hossein,
Zou Hassan Khodadadian,
Van Doan Hien,
Kolangi Miandare Hamed,
Hoseini Seyyed Morteza
Publication year - 2018
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13440
Subject(s) - biology , cyprinus , innate immune system , immune system , common carp , immunostimulant , lysozyme , downregulation and upregulation , interleukin 8 , immunology , endocrinology , andrology , inflammation , biochemistry , gene , fishery , fish <actinopterygii> , medicine
The aim of this study was to illustrate the effects of dietary loquat leaf extract ( LLE ) on the expression of some intestinal cytokines as well as serum innate immune parameters in common carp ( Cyprinus carpio ) fingerlings. The fish were fed with experimental diets supplemented with 0 (control), 0.25% (0.25 LLE ), 0.5% (0.5 LLE ) and 1% (1 LLE ) LLE over a 7‐week period. At the end of the trial, the serum lysozyme (Lys) activity, ACH 50, total Ig as well as the expression of IL 1B , IL 8 , TNF ‐alpha , LYZ and TGF ‐ β in intestine were evaluated. The results showed that administration of 0.5% or 1% LLE significantly increased serum total Ig. However, in case of serum Lys activity significant elevation was observed just in fish fed 0.5% LLE . Also, supplementation of diet with LLE significantly increased ACH 50 compared to the control group, regardless of inclusion levels. Gene expression studies revealed upregulation of TNF ‐alpha , IL 1B , IL 8 and LYZ in intestine of fish fed LLE . However, the effects varied dependent on LLE levels and the tested immune related gene. Also, in case of TGF ‐ β significant downregulation was observed just in 1% LLE treatment. In conclusion, dietary LLE supplementation significantly upregulated immune related genes in intestine and improves innate immune responses. Altogether, LLE can be recommended as fish immunostimulant in early stage of carp culture.