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Isolation of alginate lyase‐producing bacteria and screening for their potential characteristics as abalone probionts.
Author(s) -
Amin Muhamad,
Bolch Christopher C J,
Adams Mark B,
Burke Christopher M
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13383
Subject(s) - abalone , biology , lysozyme , lyase , bacteria , food science , microbiology and biotechnology , biochemistry , enzyme , fishery , genetics
This study is aimed at the isolation and screening of alginate lyase‐producing bacteria from the gastrointestinal tracts of hybrid abalone, Haliotis rubra x H. laevigata , as probiotic candidates. Six bacterial isolates were detected to produce alginate lyase. Of these, the isolate with the highest alginate‐lyase activity was identified as Enterobacter ludwigii strain EN ‐119, displaying 99% similarity of 16S rDNA sequence. Further assays indicated that E. ludwigii showed good viability and stability when it was incorporated into manufactured pellets and stored at 4°C for 7 days. The isolate also had high tolerance of high salinity (35 mg/L), low pH in simulated stomach juice (5) and to simulated intestinal juice containing surfactants such as bile salts and gastric enzymes (pepsin and trypsin). Additionally, a short, preliminary study indicated that supplementation of E. ludwigii via manufactured pellets improved the total weight gain and specific growth rate of hybrid abalone. These results suggest that E. ludwigii is a potential probiont for shortening the culture period of hybrid abalone.