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Liver histology and histomorphometry in hybrid sorubim ( Pseudoplatystoma reticulatum × Pseudoplatystoma corruscans ) reared on intensive fish farming
Author(s) -
Rodrigues Robson Andrade,
Saturnino Klaus Casaro,
Fernandes Carlos Eurico
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13325
Subject(s) - biology , histology , staining , zoology , h&e stain , haematoxylin , genetics
This study aimed to characterize the liver histology and histomorphometry in sorubim hybrid of different categories (nursery, growth and grow‐out) reared on fish farming. The categories were defined considering body weight ( BW ): nursery category ( n = 5): BW = 37.06 ± 6.00 g (31.6–45.3 g); growth category ( n = 5): BW = 310.40 ± 53.80 g (242.1–376.4 g) and grow‐out category ( n = 5): BW = 874.28 ± 27.59 g (846.2–913.1 g). Liver fragments were processed to paraffin inclusion, and sections were stained by haematoxylin and eosin (H&E), PAS (Periodic Acid Schiff) and Perl's staining to histology, histomorphometry and density volumetric of liver structures; glycogen analysis and to detect ferric irons (Fe 3+ ) respectively. The hepatosomatic index decreased between the categories ( P < 0.01). The percentage of PAS ‐positive hepatocytes in the nursery category was higher ( P < 0.05) in relation to the growth and grow‐out categories. The hepatocytes from all fish were positive to Perl's staining. The density volumetric of liver structures did not differ among categories except to blood vessels were higher ( P < 0.01) in the nursery and growth. The area (μm 2 ) and perimeter (μm) of hepatocytes, and the area (μm 2 ), perimeter (μm) and volume (μm 3 ) of the nuclei from grow‐out fish were lower ( P < 0.01) than those from the nursery and growth categories. Changes in morphometric characteristics of hepatocytes may result from metabolic changes associated with body growth surubins; therefore, these morphometric characteristics of liver tissue can be used as functional biomarkers for the assessment of fish health and nutrition status.