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The influence of diet on the grass carp intestinal microbiota and bile acids
Author(s) -
Zhang Jing,
Xiong Fan,
Wang GuiTang,
Li WenXiang,
Li Ming,
Zou Hong,
Wu ShanGong
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13312
Subject(s) - biology , deoxycholic acid , ursodeoxycholic acid , bile acid , chenodeoxycholic acid , bacteroides , glycocholic acid , bacteroidetes , fusobacteria , medicine , zoology , cholic acid , biochemistry , bacteria , 16s ribosomal rna , genetics , gene
To elucidate the influence of different diet on the intestinal microbe and bile acids, we characterized the microbiota and bile acids in the hindgut content of grass carp fed on formula feed ( FF group) or Sudan grass ( SG group). Fusobacteria and Proteobacteria were significantly more represented in FF group than in SG group whereas Bacteroidetes was significantly more abundant in SG group than in FF group. Simpson diversity was significantly higher in FF group than in SG group ( t = 2.33, P < 0.05). Chenodeoxycholic acid ( CDCA ) was the most abundant primary bile acid in the two groups, with average concentrations of 1.03 ± 0.62 and 4.44 ± 1.80 ng mg −1 in SG and FF group respectively. The most abundant secondary bile acid was deoxycholic acid ( DCA ) in SG group and ursodeoxycholic acid ( UDCA ) in FF group, with average concentrations of 0.17 ± 0.06 and 2.67 ± 0.88 ng mg −1 respectively. UDCA is significantly more abundant in FF group than in SG group, and the total bile acids were higher in FF group than in SG group. Cetobacterium and Fusobacteriaceae U114 were significantly related with the concentrations of CDCA ( r = 0.85, P < 0.05 and r = 0.82, P < 0.05 respectively) and UDCA ( r = 0.92, P < 0.01 and r = 0.92, P < 0.01 respectively). However, Bacteroides was negatively related with the concentration of UDCA ( r = −0.67, P < 0.05). Overall, there existed certain relationship between the intestinal microbes and the faecal bile acids, and they were both influenced by the diet.