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The effect of different dietary inclusion levels of rutin (a flavonoid) on some liver enzyme activities and oxidative stress indices in rainbow trout, Oncorhynchus mykiss (Walbaum) exposed to Oxytetracycline
Author(s) -
Nazeri Sepideh,
Farhangi Mehrdad,
Modarres Saghar
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13257
Subject(s) - rutin , biology , oxytetracycline , catalase , rainbow trout , flavonoid , zoology , superoxide dismutase , oxidative stress , alanine aminotransferase , medicine , food science , pharmacology , endocrinology , antioxidant , biochemistry , fish <actinopterygii> , antibiotics , fishery
The study was conducted using a completely randomized design with five treatments each with three replicates. In each treatment, 30 fish (10 per replicate) with an average body weight of 30 ± 0.6 g were randomly allocated to 60‐L tanks. Treatments consisted of a control (a diet without rutin and oxytetracycline ( OTC )), an OTC treatment (a diet free from rutin and containing 75 mg kg −1 body weight OTC ) and the ( OTC + rutin) treatments including diets with different levels of rutin, 500 (R‐500 + OTC ), 1000 (R‐1000 + OTC ) and 2000 (R‐2000 + OTC ) ppm and 75 mg kg −1 body weight per day OTC which were fed to fish for 28 days. The aspartate aminotransferase ( AST ) and alanine aminotransferase ( ALT ) activities in the blood serum after the OTC treatment were significantly ( P < 0.05) higher than in the control. After the three ( OTC +rutin) treatments, the AST and ALT activities were not significantly different from the control. The level of glucose in the blood serum was significantly lower ( P < 0.05) in all treatments as compared to the OTC treatment. Superoxide dismutase ( SOD ) and catalase ( CAT ) activities in red erythrocyte lysates were significantly ( P < 0.05) reduced after OTC treatment in comparison with the control. After the three ( OTC +rutin) treatments, the values of these indices were significantly ( P < 0.05) higher than the OTC treatment, in particular for the R‐1000 + OTC treatment. It seems that rutin, as a non‐enzymatic inhibitor, is capable of preventing damage to the liver tissue and reducing oxidative stress.