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Replacement of fishmeal by soya protein concentrate in the diets of Totoaba macdonaldi (Gilbert, 1890) juveniles: effect on the growth performance, in vitro digestibility, digestive enzymes and the haematological and biochemistry parameters
Author(s) -
TrejoEscamilla Idaly,
Galaviz Mario A,
FloresIbarra Maricela,
Álvarez González Carlos A,
López Lus M
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13225
Subject(s) - fish meal , biology , net protein utilization , zoology , chymotrypsin , protein efficiency ratio , meal , protease , food science , trypsin , fish <actinopterygii> , feed conversion ratio , biochemistry , enzyme , body weight , endocrinology , fishery
The potential of soya protein concentrate ( SPC ) as an alternative protein source in diets for Totoaba macdonaldi juveniles was evaluated. Seven isonitrogenous and isolipidic diets were formulated containing 15–100% SPC ( SPC 15, SPC 30, SPC 45, SPC 60, SPC 75, SPC 90 and SPC 100) to replace fishmeal ( FM ‐protein), and a FM ‐protein‐based diet without SPC was used as a reference diet ( RD ). Each diet was randomly assigned to triplicate groups of 20 totoaba (50 ± 1.0 g) and was fed twice daily to apparent satiation. After 60 days of experiment, effects on totoaba were evaluated. Growth performance in fish fed RD to SPC 45 was similar. The maximum FM ‐protein replacement for weight gain (g kg ABW −1 day −1 ) was estimated to be 34.17% using a broken‐line model. In vitro digestibility of fish fed RD , SPC 15 and SPC 30 was similar. Trypsin activity was higher in fish fed the RD (9.38 ± 0.52 mU × 10 −3 mg protein −1 . Chymotrypsin activity was similar in fish fed RD , SPC 15 and SPC 30. Activity of alkaline protease and phosphatase was similar in fish fed RD , SPC 15 and SPC 30. Red blood cells ( RBC ) were the highest in fish fed RD to SPC 60. Fish fed the RD , SPC 15 and SPC 30 present the highest haematocrit ( HT ) and haemoglobin ( HB ) content. Diets SPC 90 and SPC 100 presented similar MCV and MCHC . Total protein ( TP ) profile overall decreased in fish fed SPC 90 and SPC 100, pointing to nutritional hypoproteinemia due to deficient digestion and absorption. These results indicate that SPC ‐based diets could be used efficiently by totoaba with FM ‐protein replacement of less than 45%.