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Determination of selected antibiotics in farmed fish species using LC ‐ MS / MS
Author(s) -
Yipel Mustafa,
Kürekci Cemil,
Tekeli İbrahim O,
Metli Murat,
Sakin Fatih
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13209
Subject(s) - oxytetracycline , enrofloxacin , biology , tilmicosin , antibiotics , florfenicol , tetracycline , penicillin , veterinary medicine , rainbow trout , flumequine , fish farming , aquaculture , fishery , microbiology and biotechnology , fish <actinopterygii> , ciprofloxacin , medicine
The aim of the study was to monitor the occurrence of 37 antibiotics from different classes including fluoroquinolone, tetracycline, macrolide, sulphonamide, penicillin, amphenicol and cephalosporin in three different farmed fish species ( n = 75; rainbow trout, see bass, gilthead see bream) from five cities in Turkey. A method for LC ‐ MS / MS was optimized and validated to assess these antibiotics. As a result, 25 samples showed contamination with at least one antibiotic residue. The most detected antibiotics were norfloxacin ( NOR ) (18.7%), marbofloxacin ( MAR ) (12%), tetracycline ( TC ) (10.7), danofloxacin ( DAN ) (9.3%), oxytetracycline ( OTC ) (9.3%), tilmicosin ( TLM ) (8%) and enrofloxacin ( ENR ) (6.7%). A total of 17.3% of samples examined were found to be contaminated with residues exceeding the maximum residue limits ( MRL s). The results indicate that antibiotics are frequently used in aquaculture production without the lack of knowledge. This study also revealed that the consumption of farmed fish could produce a public health concern as the level of residues for some antibiotics was much higher than MRL s.