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Effect of dietary supplementation of palm fruit extracts on the transcriptomes of growth, antioxidant enzyme and immune‐related genes in common carp (Cy prinus carpio ) fingerlings
Author(s) -
Hoseinifar Seyed Hossein,
Dadar Maryam,
Khalili Mohsen,
Cerezuela Rebeca,
Esteban Maria Ángeles
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13192
Subject(s) - biology , common carp , cyprinus , glutathione peroxidase , glutathione reductase , antioxidant , carp , immune system , glutathione , gene , endocrinology , medicine , enzyme , biochemistry , immunology , fishery , fish <actinopterygii>
This study investigates the effects of date palm extracts [DPE] ( Phoenix dactylifera L. Arecaceae ) on growth, immune function and antioxidant system in common carp fingerlings ( Cyprinus carpio ). One hundred and twenty fish (4.06 ± 0.13 g) were divided into six groups fed on control diet or diets containing 200 mL kg −1 DPE for 8 weeks. At the end of feeding trial, the expression of different genes was measured. Selected genes were grouped into three categories: growth factor genes in brain and liver [growth hormone (GH), insulin‐like growth factors I and II (IGF‐I and IGF‐II), antioxidant‐related genes in liver [glutathione S‐transferase‐alpha (GST‐α), glutathione reductase (GR) and glutathione peroxidase genes (GPX)] and immune‐related genes in head kidney [interleukin‐8 (IL‐8), interleukin‐10 (IL‐10) and transforming growth factor‐beta (TGF‐β) genes]. The relative expression of the growth‐related genes in fish fed DPE showed no significant increase compared to control group ( P > 0.05). On the other hand, DPE altered the expression of genes encoding antioxidants enzymes in liver of fingerlings which was statistically significant with respect to the control samples in case GPX ( P < 0.05). Also, DPE caused remarkable increases in the expression of the immune‐related genes (IL‐8, IL‐10 and TGF‐β) analysed on head kidney of common carp fingerlings compared to the control group ( P < 0.05). In conclusion, it can be suggested that administration of DPE in early stages of common carp culture can promote immune efficacy and increase the antioxidant activity.