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In vitro and in vivo selection of probiotic purple nonsulphur bacteria with an ability to inhibit shrimp pathogens: acute hepatopancreatic necrosis disease‐causing Vibrio parahaemolyticus and other vibrios
Author(s) -
Chumpol Supaporn,
Kantachote Duangporn,
Rattanachuay Pattamarat,
Vuddhakul Varaporn,
Nitoda Teruhiko,
Kanzaki Hiroshi
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13149
Subject(s) - shrimp , biology , litopenaeus , probiotic , vibrio parahaemolyticus , microbiology and biotechnology , vibrio , bacteria , fishery , genetics
Shrimp cultivation has been faced with huge losses in productivity caused by infectious shrimp pathogenic vibrios, especially Vibrio parahaemolyticus that causes acute hepatopancreatic necrosis disease ( AHPND ). Hence, purple nonsulphur bacteria ( PNSB ) were isolated from shrimp ponds for investigating their abilities to control shrimp pathogenic Vibrio spp. and their use as probiotics for sustainable shrimp cultivation. Based on their probiotic properties, strains S3W10 and SS 15 were selected because of their strong abilities to produce amylase, gelatinase and vitamin B12. However, only three PNSB strains ( SS 15, TKW 17 and STW 181) strongly inhibited V. harveyi _ KSAAHRC and V. vulnificus _ KSAAHRC including V. parahaemolyticus AHPND strains by secreting antivibrio compounds. Four selected PNSB also grew in the presence of pancreatic enzymes, and they were identified as Rhodobacter sphaeroides for strains S3W10, SS 15 and TKW 17 and Afifella marina for strain STW 181. The effects of a mixed culture were also investigated as follows: T1 (S3W10 + SS 15), T2 (S3W10 + TKW 17) and T3 (S3W10 + STW 181) on postlarval white shrimp ( Litopenaeus vannamei ) for 60 days by comparison with a control. All three probiotic PNSB sets significantly improved the digestive enzyme activities and shrimp growth with their proliferation in shrimp gastrointestinal tract although the shrimp survival was not significantly different. They also significantly reduced the cumulative mortality of shrimp exposed to a virulent AHPND strain ( V. parahaemolyticus SR 2). This is the first to conclude that selected probiotic PNSB strains have great potential to be used for shrimp cultivation to control vibrios including AHPND strains.