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Spawning of female Black tiger shrimp ( Penaeus monodon ) is not impacted by muscle injection of ds RNA targeted to gill‐associated virus
Author(s) -
Sellars Melony J,
Rao Min,
Cowley Jeff A
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13123
Subject(s) - broodstock , shrimp , penaeus monodon , biology , spawn (biology) , hatchery , fishery , decapoda , penaeus , rna silencing , zoology , andrology , aquaculture , anatomy , rna , crustacean , gene , biochemistry , fish <actinopterygii> , medicine , rna interference
The ability of domesticated Penaeus monodon , Black Tiger shrimp, to spawn following tail‐muscle injection of ds RNA was examined. Ablated domesticated female broodstock infected subclinically with gill‐associated virus ( GAV ) were injected with saline or a cocktail of five‐ds RNA s targeting different regions in the GAV ORF 1a/1b gene. To track changes in GAV infection loads, TaqMan real‐time PCR was used to quantify mean viral RNA amounts in each of three pleopod clips collected at the time of injection (Day 0) and either immediately after a female spawned or on Day 11 when the trial was terminated. Over the trial, 4 of 19 (21%) saline‐injected shrimp spawned and 12 of 25 (48%) ds RNA ‐injected shrimp spawned, with one spawning twice. Egg numbers varied from 25 600 to 459 800 for the saline‐injected shrimp and from 4900 to 213 900 for the ds RNA ‐injected shrimp. Of these, one of the four egg batches hatched from saline‐injected shrimp and 9 of the 13 egg batches hatched from ds RNA ‐injected shrimp. While variable, egg numbers and hatch rates recorded were typical of those obtained from domesticated broodstock at the commercial hatchery and particularly among females previously spawned. Mean GAV RNA amounts detected in pleopod samples increased in five of the eight saline‐injected shrimp tested by 1.6–227.4‐fold and decreased in 12 of the 15 ds‐ RNA ‐injected shrimp tested by −1.1 to −45.1‐fold. The study demonstrated that tail‐muscle injection of GAV ‐specific ds RNA does not adversely impact the ability of P. monodon to spawn.