Dietary selenium requirement for on‐growing gibel carp ( Carassius auratus gibelio var. CAS III)

A 12‐week growth trial was conducted in a flow‐through system to determine dietary selenium (Se) requirement for on‐growing gibel carp (initial body weight: 76.2 ± 0.05 g, mean ± SEM). Selenomethionine was supplemented to the basal diet to formulate seven semi‐purified diets containing 0.26, 0.58, 0.72, 1.14, 1.34, 1.73 and 2.09 mg Se kg −1 diet. The results showed that plasma superoxide dismutase (SOD) activity significantly increased when fish were fed with 0.58 mg Se kg −1 diet ( P  <   0.05) and then decreased at 2.09 mg Se kg −1 diet ( P  <   0.05). Plasma T‐AOC activity was higher in fish fed with 0.72 mg Se kg −1 diet ( P  <   0.05) and plasma malondialdehyde (MDA) was higher in fish fed with 0.26 mg Se kg −1 diet ( P  <   0.05). When fish were fed 1.14 mg Se kg −1 diet, hepatic GSH‐Px, T‐AOC, GSH and CAT activities were significantly higher than those fed with 0.26 mg Se kg −1 diet ( P  <   0.05). Hepatic superoxide dismutase (SOD) activity was higher at 1.34 mg Se kg −1 diet ( P  <   0.05). Fish liver Se concentrations were significantly higher when fed with 0.72 mg Se kg −1 diet ( P  <   0.05) and then kept constant when Se ≥ 0.72 mg kg −1 ( P  >   0.05). Whole‐body and muscle Se concentrations were higher when fed with 1.34 mg Se kg −1 diet ( P  <   0.05) and kept a plateau when Se ≥ 1.34 mg kg −1 ( P  >   0.05). In conclusion, based on broken‐line regression of hepatic Se concentrations, hepatic SOD activity and hepatic T‐AOC activity, dietary Se requirements for on‐growing gibel carp was 0.73 mg kg −1 , 1.12 mg kg −1 and 1.19 mg kg −1 diet respectively.