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In vitro storage of fish oocytes: effect of storage temperature, media conditions and storage duration on fertilization and larval hatchability of Indian major carp, rohu ( Labeo rohita )
Author(s) -
Mishra Gayatri,
Patra Sunita,
Dash Santosh Kumar,
Verma Dhananjay Kumar,
Routray Padmanav
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13086
Subject(s) - biology , hatching , carp , human fertilization , labeo , larva , in vitro fertilisation , zoology , fishery , andrology , fish <actinopterygii> , anatomy , botany , embryo , medicine
The viability of matured oocytes stored in vitro were assessed using carp ovarian fluid ( OF ) and artificial carp ovarian fluid ( ACOF ) under different temperature regimes (4, 24, 26, 28 and 30°C) for different storage durations (0, 60, 120, 150 and 180 min). Significantly higher fertilization (74%) was achieved when the oocytes were stored using ACOF and 65% in OF after 180 min at 28°C. Similarly the hatching rates of larvae were significantly higher in the ACOF and OF , that is, 64% and 47%, respectively, after 180 min of storage. The oocytes kept in the storage containers with ACOF having 65% moisture level showed a significantly higher fertilization rate than the 59% moisture level. This study demonstrated that unfertilized matured oocytes (eggs) of rohu can be stored in vitro for 180 min without compromising the viability (fertilization and hatching) to a great extent in OF and ACOF .