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Antibacterial activity of immobilized silver nanoparticles on TEPA ‐Den‐SiO 2 against shrimp pathogen, Vibrio sp. Persian1
Author(s) -
Sarkheil Mehrdad,
Sourinejad Iman,
Mirbakhsh Maryam,
Kordestani Davood,
Johari Seyed Ali
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13048
Subject(s) - shrimp , silver nanoparticle , antibacterial activity , nuclear chemistry , vibrio , seawater , microbiology and biotechnology , bacteria , biology , materials science , food science , nanoparticle , chemistry , nanotechnology , fishery , genetics , ecology
In this study, we prepared silver nanoparticles immobilized onto silica sand beads as an antibacterial material against pathogenic luminous Vibrio sp. Persian1. Silica beads were modified with 3‐aminopropyltriethoxysilane ( APTES ), cyanuric chlorid and tetraethylene pentamine, and silver nanoparticles were generated in various concentrations (0.5, 1, and 2 mM g −1 of silica beads) of Ag NO 3 on the surface using chemical reduction. Ag/ TEPA ‐Den‐SiO 2 samples were characterized by TEM , FE ‐ SEM / EDS , FT ‐ IR and ICP OES and their antibacterial activity assayed by zone of inhibition and test tube tests against pathogenic Vibrio sp. The results of the zone inhibitory test revealed that all the Ag/ TEPA ‐Den‐SiO 2 samples had an antibacterial effect against luminous Vibrio sp. Persian1. In addition, the tube test results showed 100% killing of bacteria in 2 h contact period. Ag/ TEPA ‐Den‐SiO 2 samples maintained their antibacterial activity after 14‐day immersion in seawater by slow release of silver ions. These results suggest that Ag/ TEPA ‐Den‐SiO 2 substrates could be effective antibacterial materials for disinfection of seawater used to culture Penaeid shrimp larvae.