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Development of real‐time and quantitative QCM immunosensor for the rapid diagnosis of Aeromonas hydrophila infection
Author(s) -
Hong SungRok,
Kim MyungSug,
Jeong HyunDo,
Hong Suhee
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13039
Subject(s) - aeromonas hydrophila , quartz crystal microbalance , detection limit , pathogenic bacteria , biology , pathogen , biosensor , bacteria , microbiology and biotechnology , fish <actinopterygii> , aeromonas , chromatography , chemistry , biochemistry , adsorption , fishery , genetics , organic chemistry
Since bacterial infection cause a significant economic loss in fish farms, it is necessary to develop rapid diagnostic tools. Interests on label‐free biosensors have been raised for the rapid detection of aquatic pathogenic bacteria but have not been extensively studied yet. Here we report a quartz crystal microbalance ( QCM ) immunosensor system for the rapid and simple detection of Aeromonas hydrophila , a pathogen for fish and human, in comparison with a conventional indirect ELISA method. In QCM immunosensor system, an antibody against A. hydrophila was covalently cross‐linked to the gold surface of sensor chip and bacterial attachment was monitored as real‐time frequency shifts within 5 min. The frequency shifts were very positively related to the amounts of bacterial cells between 6.25 and 100 μg corresponding to 6 × 10 6 to 10 8 CFU with a high specificity. The QCM immunosensor was also able to detect bacterial cells in fish tissue extract in a dose‐dependent manner. Indirect ELISA also showed the dose‐dependent reaction and the amplified signal may allow a lower detection limit. However, QCM immunosensor system showed a more linear and reliable standard curve with R 2 value of almost 1 (0.9999). Moreover, detection of the bacteria was much quicker and simpler.