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Characterization of digestive enzymes protease and alpha‐amylase activities in the thick‐lipped grey mullet ( Chelon labrosus , Risso 1827)
Author(s) -
Pujante Isabel Maria,
DíazLópez Manuel,
Mancera Juan Miguel,
Moyano Francisco Javier
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13038
Subject(s) - biology , pepsin , trypsin , lipase , amylase , proteases , digestive enzyme , protease , chymotrypsin , enzyme , biochemistry , digestion (alchemy) , serine protease , chromatography , chemistry
Activities of pepsin, trypsin, chymotrypsin, alpha‐amylase and lipase, as well as their optima and stability to pH and temperature, were determined in digestive extracts of thick‐lipped grey mullet, Chelon labrosus, of three different sizes: Group 1 (45.2 ± 3.0 g), Group 2 (180.9 ± 4.2 g), and Group 3 (328.5 ± 43.3 g). SDS ‐ PAGE zymograms were also used to assess the role of serine proteases in the digestive tract of C. labrosus . On the other hand, possible changes in the digestive enzyme profile of C. labrosus during development were observed, with a comparatively lower pepsin activity, higher activities of alkaline proteases and alpha‐amylase and no lipase activity recorded in pre‐adult specimens. It is suggested that these variations are linked to the changes in diet composition with age, moving from a partly carnivorous to a more herbivorous feeding habit.