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Anaesthetic and antioxidant effects of Myrcia sylvatica (G. Mey.) DC . and Curcuma longa L. essential oils on tambaqui ( Colossoma macropomum )
Author(s) -
Saccol Etiane M H,
Toni Cândida,
Pês Tanise S,
Ourique Giovana M,
Gressler Luciane T,
Silva Lenise V F,
Mourão Rosa H V,
Oliveira Ricardo B,
Baldisserotto Bernardo,
Pavanato Maria A
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13034
Subject(s) - antioxidant , biology , glutathione peroxidase , glutathione , glutathione reductase , lipid peroxidation , catalase , oxidative stress , sedation , food science , pharmacology , biochemistry , enzyme
Anaesthetic substances are necessary to reduce fish stress during aquaculture activities. The objectives of this study were: (i) to determine the efficacy of essential oils ( EO s) of Myrcia sylvatica ( EOMS ) and Curcuma longa ( EOCL ) as anaesthetics for Colossoma macropomum and (ii) to evaluate the effects of rapid anaesthesia and long‐term sedation (6 h) with these oils. Therefore, the main primary stress indicator (cortisol) and secondary factors (biochemical indices, hepatic metabolism, oxidative biomarkers) were measured. Sedation with the EOCL resulted in lower cortisol levels compared to control group. Total cholesterol levels were lower in fish sedated with EOMS than in control. Lactate levels were higher in fish anaesthetized with both EO s and sedated with EOCL compared to control. Both EO s increased hepatic glycogen levels after anaesthesia and EOMS increased this parameter after sedation compared to control. Anaesthesia and sedation with EO s resulted in lower levels of lipid peroxidation ( LPO ) compared to control. In turn, the activity of some antioxidant enzymes evaluated (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione‐S‐transferase), the content of non‐protein thiols and total reactive antioxidant potential were higher in tissues of fish anaesthetized and sedated with EO s compared to control. This induction of antioxidant capacity in the tissues could be due to the antioxidant property exerted by these EO s. Thus, EOMS and EOCL are recommended for anaesthesia and sedation of fish because in spite of inducing anaerobic metabolism, these EO s did not alter most biochemical parameters, reduced the LPO and increased the antioxidant capacity in vital tissues.