Premium
Enhancement and confirmation of white spot syndrome virus detection using monoclonal antibody specific to VP 26
Author(s) -
Vaniksampanna Akapon,
Longyant Siwaporn,
Wangman Pradit,
Sithigorngul Paisarn,
Chaivisuthangkura Parin
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.13007
Subject(s) - white spot syndrome , monoclonal antibody , shrimp , biology , epitope , blot , virology , microbiology and biotechnology , antibody , virus , gene , immunology , biochemistry , fishery
A portion of the VP 26 gene ( VP 26F109) encoding a structural protein of white spot syndrome virus was expressed, purified by SDS ‐ PAGE and used for immunization of Swiss mice for monoclonal antibody ( MA b) production. Three groups of MA bs specific to different epitopes on VP 26 were selected; these MA bs can be used to detect natural WSSV infection in Penaeus vannamei using dot blotting, Western blotting or immunohistochemistry without cross‐reaction with other shrimp tissues or other common shrimp viruses. The detection sensitivity of the MA bs was ranged 7–14 fmole per spot of the rVP 26F109 as determined using dot blotting. A combination of three MA bs specific to VP 26 with MA bs specific to VP 28, VP 19 and ICP 11 increased the detection sensitivity of WSSV during early infection. Therefore, the MA bs specific to VP 26 could be used to confirm and to enhance the detection sensitivity for WSSV infection in shrimp with various types of antibody‐based assays.