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Comparison of immune responses and antioxidant status of different generations of growth‐selected P ortunus trituberculatus families
Author(s) -
Ren Xianyun,
Gao Baoquan,
Liu Xianxi,
Li Jian,
Liu Ping
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12973
Subject(s) - hepatopancreas , portunus trituberculatus , hemolymph , biology , superoxide dismutase , inbreeding , antioxidant , glutathione peroxidase , catalase , prophenoloxidase , immune system , biochemistry , immunology , innate immune system , ecology , population , demography , sociology
The purpose of this study was to investigate the effects of inbreeding on the immune responses and antioxidant status of P ortunus trituberculatus juveniles. Results showed that inbreeding affected the total haemocyte count, and phagocytic, pro‐phenoloxidase (propo), phenoloxidase and antibacterial activities decreased after the seventh generation. Antioxidant status showed a similar pattern: total antioxidant capacity, superoxide dismutase ( SOD ) activity and GSH / GSSG in the cell‐free haemolymph and hepatopancreas decreased, while catalase activity in the cell‐free haemolymph increased ( P < 0.05). There were no significant differences in α 2 ‐macroglobulin and bacteriolytic activities in the cell‐free haemolymph and glutathione peroxidase activity in the cell‐free haemolymph and hepatopancreas among nine inbreeding generations. Gene expression levels of pro PO and crustin in haemocytes and SOD in haemocytes and hepatopancreas also decreased significantly as the inbreeding generations increased. The results suggest that a high level of inbreeding could severely reduce the physiological health of P . trituberculatus . Our obtained data would be particularly useful for P . trituberculatus breeding programmes.