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Effect of dietary chitosan oligosaccharide complex with Ce ( IV ) on growth, immunity and disease resistance against Vibrio splendidus of sea cucumber, Apostichopus japonicas
Author(s) -
Wang Yanlong,
Xu Wei,
Zuo Rantao,
Zhou Huihui,
Bai Yang,
Mai Kangsen,
Wang Dongfeng,
Ai Qinghui
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12957
Subject(s) - sea cucumber , biology , apostichopus japonicus , salinity , respiratory burst , zoology , immunity , food science , chitosan , alkaline phosphatase , microbiology and biotechnology , immune system , biochemistry , immunology , enzyme , ecology
An 8‐week feeding trial was conducted to investigate the effects of dietary chitosan oligosaccharide complex with cerium (Ce IV) ( COS ‐Ce) on growth performance, nonspecific immunity and disease resistance of sea cucumber, Apostichopus japonicas . Five isonitrogenous (18.6%) and isolipidic (1.1%) practical diets were formulated with graded level of COS ‐Ce (0, 150, 300, 600 and 1200 mg kg −1 dry feed), which were named as COS ‐Ce/0, COS ‐Ce/150, COS ‐Ce/300, COS ‐Ce/600, COS ‐Ce/1200 respectively. Each diet was allocated to four replicates of sea cucumbers (Initial weight: 6.72 ± 0.02 g). Sea cucumbers were fed to apparent satiation once daily (19:00 hours) for 56 days. During the experiment, water temperature was kept at 16 ± 0.5°C, pH 7.8–8.2, dissolved oxygen beyond 5 mg L −1 , ammonia nitrogen below 0.5 mg L −1 and salinity from 30‰ to 31‰. Results showed that the specific growth rate of sea cucumbers was significantly higher in COS ‐Ce/600 than that in other four treatments. Activities of phagocytosis, respiratory burst, acid phosphatase and alkaline phosphatase in COS ‐Ce/600 were significantly higher than that in COS ‐Ce/0 ( P < 0.05) respectively. On the contrary, cumulative mortality was the lowest in COS ‐Ce/600 following 14 days exposure to Vibrio splendidus ( P < 0.05). In conclusion, these results confirmed that dietary COS ‐Ce had beneficial effects on growth performance, nonspecific immunity and disease resistance of sea cucumber.