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Effect of live algae used as green water on survival, growth, behaviour, ontogeny and bacterial profile of lobster larvae ( H omarus americanus M ilne E dwards)
Author(s) -
Haché Rémy,
Dumas André,
Thumbi David,
Forward Benjamin S,
Mallet Martin
Publication year - 2017
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12905
Subject(s) - biology , homarus , american lobster , algae , larva , live food , isochrysis galbana , aquaculture , green algae , fishery , food science , ecology , botany , crustacean , fish <actinopterygii>
Abstract Green water is a technique commonly used in aquaculture, that consists of adding live algae in the water culture and its benefits have been shown for several species. Several hypotheses exist to explain the benefits of green water: increase in nutritional value; action as a probiotic; increase in contrast to reveal preys for larvae; or increase in predator behaviour of larvae. Green water produced with a mix of strains I sochrysis galbana and C haetoceros muelleri (50:50, cells:cells) applied in four different ways was tested. The survival and the growth of American lobster ( H omarus americanus ) between stage I and stage IV post‐larvae were not affected by the addition of live algae. The lipid classes were not affected by the addition of algae and limited variation was observed in the fatty acids and bacterial profiles. Furthermore, the green water techniques had a limited effect on the behaviour of post‐larvae stage IV lobster at releasing. Behaviour was mostly affected by the age of post‐larvae. The bacteria L ewinella sp., L eucothrix sp. and T hiothrix sp. appeared to represent a common and core component of Stage IV lobster post‐larvae microflora. The results show that the algae do not increase either nutritional value or feed intake of the lobster larvae. Probiotic effect may be more important when larvae are raised in a close system where potential bacterial pathogens could have more chances to colonize the culture. Also, the dark green colour of the larval tank used in this study may have mimicked the effect of green water in the control group. Biochemical results suggest that dietary supplementation with phospholipids and DHA is needed in a lobster hatchery using frozen A rtemia and our open formula Dry mix .