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Nutritional and biochemical studies on feeding of hydrolysed and unhydrolysed detoxified Jatropha curcas protein isolate in common carp fingerlings
Author(s) -
Latif Sajid,
Kumar Vikas,
Stadtlander Timo,
Makkar Harinder P S,
Becker Klaus
Publication year - 2016
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12838
Subject(s) - biology , zoology , carp , common carp , albumin , cyprinus , fish meal , jatropha , food science , biochemistry , fish <actinopterygii> , fishery , biodiesel , catalysis
Detoxified Jatropha protein isolate ( DJPI ), prepared from Jatropha seed cake was hydrolysed using Alcalase 2.4L FG . Both DJPI and hydrolysed DJPI ( HDJPI ) were evaluated as a fish meal ( FM ) replacement in common carp ( Cyprinus carpio L.) diets. The peptide concentration in HDJPI was determined using matrix‐assisted laser desorption ionization‐time of flight mass spectrometer ( MALDI ‐ TOF ). Twenty‐two peptides with molecular mass ranging from m/z 906.455 to 1774.943 Da were identified. Fingerlings (45; av. wt. 10.1 ± 0.4 g) were randomly distributed in five groups with three replicates. An 8‐week feeding experiment was conducted to compare the nutritional quality of the HDJPI , DJPI and FM . Fish were fed on iso‐nitrogenous diets: control ( FM based protein), J 50 (50% of FM protein replaced by DJPI ), J 75 (75% of FM protein replaced by DJPI ), JH 50 (50% of FM protein replaced by HDJPI ) and JH 75 (75% of FM protein replaced by HDJPI ). Growth performance was higher for FM fed group compared with the other groups, while nutrient utilization parameters were similar for control and J 50 groups and superior ( P < 0.05) to J 75 and both the HDJPI fed groups. No variation ( P > 0.05) was observed in blood albumin and hepatosomatic index among all different groups. Blood urea nitrogen, calcium, sodium and potassium ions in the blood were similar among all five groups.