Chronic effects of NeemAzal on biochemical parameters of grass carp, Ctenopharyngodon idella

The aim of this study was to assess the chronic effects of a bio‐pesticide (NeemAzal) on some biochemical parameters of grass carp ( C tenopharyngodon idella ) under laboratory conditions. At first, 96 h LC 50 of the pesticide was determined using probit analysis software and was found to be 0.73 mg L −1 of the active ingredient (azadirachtin) of the pesticide. Fresh fish were then divided into three treatments and a control and were exposed to sublethal concentrations (10%, 20% and 30% of the 96 h LC 50 value) of the pesticide for a month. The biochemical examination of the blood showed a remarkable decrease in values of protein, albumin, AST , ALT , ALP and LDH ( P  < 0.05).