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Effect of poly‐β‐hydroxybutyrate on growth, enzyme activity and intestinal microbial community of C hinese mitten crab, E riocheir sinensis ( M ilne‐ E dwards) juveniles
Author(s) -
Sui Liying,
Ma Guannan,
Lu Wanlong,
Deng Yuangao,
Bossier Peter,
De Schryver Peter
Publication year - 2016
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12817
Subject(s) - eriocheir , chinese mitten crab , biology , alkaline phosphatase , food science , lipase , acid phosphatase , digestive enzyme , zoology , moulting , aeromonas hydrophila , superoxide dismutase , scylla paramamosain , hemolymph , biochemistry , bacteria , enzyme , ecology , larva , genetics , gene
Poly‐β‐hydroxybutyrate ( PHB ) is microbial carbon and energy storage polymer, which can be degraded into water‐soluble β‐hydroxybutyric acid in the gastrointestinal tract of aquatic animals. A 60‐day culture experiment was performed using C hinese mitten crab, E riocheir sinensis (Milne‐Edwards) juveniles with an average initial body weight of 0.74 ± 0.06 g which were fed a diet supplemented with 0%, 0.5%, 1%, 3% or 5% PHB . A PHB dietary supplementation of 1% and 3% significantly improved the body weight gain, moulting frequency and concomitantly reduced 2nd–3rd moulting intervals of the crabs ( P < 0.05). The dietary PHB level positively related to hepatopancreatic pepsin, trypsin and lipase activity ( P < 0.05). Increasing the dietary PHB also improved total superoxide dismutase activity, but reduced alkaline phosphatase and acid phosphatase activity in the serum of hemolymph ( P < 0.05). A 16S rRNA gene analysis by denaturing gradient gel electrophoresis indicated that PHB supplementation led to a significantly higher range‐weighted richness, diversity and evenness of the gut bacterial community when dosed at 3% in the feed. The beneficial effects of PHB are discussed in terms of immune defense, metabolism and gut microbiota of the crabs.