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Molecular characterization of calreticulin gene in mud crab Scylla paramamosain (Estampador): implications for the regulation of calcium homeostasis during moult cycle
Author(s) -
Xu Binpeng,
Long Chen,
Dong Weiren,
Shao Qingjun,
Shu Miaoan
Publication year - 2016
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12781
Subject(s) - scylla paramamosain , hepatopancreas , biology , moulting , calreticulin , endoplasmic reticulum , microbiology and biotechnology , gene , ecology , genetics , larva
Calreticulin ( CRT ), a well conserved endoplasmic reticulum‐resident protein for Ca 2+ ‐binding, is widely expressed in multicellular eukaryotes. CRT plays a key role in many cellular processes, including Ca 2+ homeostasis. To address the role of CRT underlying the Ca 2+ homeostasis alternation during the mineralization cycle of the mud crab ( Scylla paramamosain ), we cloned the full‐length cDNA of CRT gene ( Sp CRT ) from S. paramamosain , and its protein contains all signature domains of CRT . Tissue distribution showed the Sp CRT transcript was far more abundant in hepatopancreas than in others. Meanwhile, seven moult stages of the crab were characterized by observing the third maxilliped under microscope and clear uropod images were presented. During the moult cycle, the total calcium levels in the hepatopancreas were determined using ICP ‐ AES . It decreased significantly at stage A and B ( p < 0.05), and was relatively steady between inter‐moult C and early pre‐moult D 0 ( p > 0.05). It was then gradually increased thereafter by nearly 274.2% until stage E (compared with stage C) ( p < 0.05). Additionally, the expression of Sp CRT in the hepatopancreas was the lowest in the moult stage E and post‐moult stage A, and then it maintained at a high level in other stages. Taken together, the relative retard timing of calcium incrument corresponding to the higher expression of Sp CRT may suggest CRT plays a critical role in Ca 2+ storage in hepatopancreas during the moult cycle of crustaceans.