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Extracts of O liviera decumbens and S atureja khuzestanica as immunostimulants affect some innate immunity indices of C yprinus carpio against A eromonas hydrophila infection
Author(s) -
Alishahi Mojtaba,
Halimi Mostafa,
Khansari Alireza,
Yavari Vahid
Publication year - 2016
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.12742
Subject(s) - biology , aeromonas hydrophila , lysozyme , cyprinus , microbiology and biotechnology , immunity , immune system , common carp , carp , immunostimulant , innate immune system , immunology , fish <actinopterygii> , fishery , biochemistry
In this study, the effect of extracts of two herbs ( O liviera decumbens and S atureja khuzestanica ) on immune response of carp was investigated. At the beginning of experiment, fish were divided to two groups including vaccinated (using vaccine developed against A . hydrophila ) and non‐vaccinated. Fish in both groups were fed diets containing O . decumbens and S . khuzestanica and combination of two herbal extracts for 5 weeks. Control fish (negative control) and fish vaccinated only (positive control) were fed basal diets without supplements of herbal extracts. Lysozyme activity, antibody titre, complement activity and bactericidal activity in serum were measured. After 5 weeks feeding, fish were infected with A . hydrophila and mortalities were recorded. In both experimental groups, no significant differences were found in terms of alternative haemolytic complement ( ACH 50) activity and antibody titres of Serum. In non‐vaccinated fish group, lysozyme and bactericidal activity of fish fed S . khuzestanica or combination of O . decumbens and S . khuzestanica was higher compared with control and other experimental treatments. In vaccinated fish group, the lysozyme and bactericidal activity was not significant in all treatments compared with control group. The results of this study showed that feeding non‐vaccinated and vaccinated carp with only S . khuzestanica or in combination with O . decumbens enhance only some immunity indices including lysozyme and bactericidal activity in non‐vaccinated fish and these extracts have no immunological stimulatory role on vaccinated individuals.